Transforming Agriculture, Perennially
Donate

Scientific Publications

|

Nitrogen use efficiency is regulated by interacting proteins relevant to development in wheat

Author: Lei Lei, Genqiao Li, Hailin Zhang, Carol Powers, Tilin Fang, Yihua Chen, Shuwen Wang, Xinkai Zhu, Brett F Carver, Liuling Yan

Publication: Plant Biotechnology Journal

Summary:

Wheat (Triticum aestivum) has low nitrogen use efficiency (NUE). The genetic mechanisms
controlling NUE are unknown. Positional cloning of a major quantitative trait locus for N-related agronomic traits showed that the vernalization gene TaVRN-A1 was tightly linked with TaNUE1, the gene shown to influence NUE in wheat. Because of an Ala180/Val180 substitution, TaVRN-A1a and TaVRN-A1b proteins interact differentially with TaANR1, a protein encoded by a wheat orthologue of Arabidopsis nitrate regulated 1 (ANR1). The transcripts of both TaVRN-A1 and TaANR1 were down-regulated by nitrogen. TaANR1 was functionally characterized in TaANR1::RNAi transgenic wheat, and in a natural mutant with a 23-bp deletion including 10-bp at the 50 end of intron 5 and 13-bp of exon 6 in gDNA sequence in its gDNA sequence, which produced transcript that lacked the full 84-bp exon 6. Both TaANR1 and TaHOX1 bound to the Ala180/ Val180 position of TaVRN-A1. Genetically incorporating favourable alleles from TaVRN-A1, TaANR1 and TaHOX1 increased grain yield from 9.84% to 11.58% in the field. Molecular markers for allelic variation of the genes that regulate nitrogen can be used in breeding programmes aimed at improving NUE and yield in novel wheat cultivars.

Access full article.

Share On: Share on Facebook Share on Twitter Select other ways to share